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Total 109548 results found since Jan 2013.

Overexpression of porcine lipoprotein-associated phospholipase A2 in swine.
Abstract Lipoprotein-associated phospholipase A 2 (Lp-PLA2) is associated with the risk of vascular disease. It circulates in human blood predominantly in association with low-density lipoprotein cholesterol (LDL-C) and hydrolyses oxidized phospholipids into pro-inflammatory products. However, in the mouse circulation, it predominantly binds to high-density lipoprotein cholesterol (HDL-C) and exhibits anti-inflammatory properties. To further investigate the effects of Lp-PLA2 in the circulation, we generated over-expressed Lp-PLA2 transgenic swine. The eukaryotic expression plasmid of porcine Lp-PLA2 which driven ...
Source: Biochemical and Biophysical Research communications - August 14, 2015 Category: Biochemistry Authors: Tang X, Wang G, Liu X, Han X, Li Z, Ran G, Li Z, Song Q, Ji Y, Wang H, Wang Y, Ouyang H, Pang D Tags: Biochem Biophys Res Commun Source Type: research

Molecular symmetry-constrained systematic search approach to structure solution of the coiled-coil SRGAP2 F-BARx domain
SRGAP2 (Slit – Robo GTPase-activating protein 2) is a cytoplasmic protein found to be involved in neuronal branching, restriction of neuronal migration and restriction of the length and density of dendritic postsynaptic spines. The extended F-BAR (F-BARx) domain of SRGAP2 generates membrane protrusions when expressed in COS-7 cells, while most F-BARs induce the opposite effect: membrane invaginations. As a first step to understand this discrepancy, the F-BARx domain of SRGAP2 was isolated and crystallized after co-expression with the carboxy domains of the protein. Diffraction data were collected from two significantly n...
Source: Acta Crystallographica Section D - November 28, 2016 Category: Biochemistry Authors: Sporny, M. Guez-Haddad, J. Waterman, D.G. Isupov, M.N. Opatowsky, Y. Tags: exhaustive search SRGAP2 F-BAR coiled coil molecular replacement research papers Source Type: research

Differential secretome profiling of swine tracheal cell line infected with mycoplasmas of swine respiratory tract.
Abstract Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetically similar. However, M. hyopneumoniae causes porcine enzootic pneumonia, while M. flocculare is a commensal bacterium. M. hyopneumoniae and M. flocculare do not penetrate their host cells, and secreted proteins are important for bacterium-host interplay. Thus, the secretomes of a swine trachea cell line (NPTr) infected with M. hyopneumoniae 7448 (a pathogenic strain), M. hyopneumoniae J (a non-pathogenic strain) and M. flocculare were compared to shed light in bacterium-host interactions. Medium from the cultures was collected, and secreted p...
Source: Journal of Proteomics - August 31, 2018 Category: Biochemistry Authors: Dos Anjos Leal Zimmer FM, Paludo GP, Moura H, Barr JR, Ferreira HB Tags: J Proteomics Source Type: research

Two separate pathways underlie NADH and succinate oxidation in swine heart mitochondria: Kinetic evidence on the mobile electron carriers
Biochim Biophys Acta Bioenerg. 2023 Apr 12:148977. doi: 10.1016/j.bbabio.2023.148977. Online ahead of print.ABSTRACTWe have investigated NADH and succinate aerobic oxidation in frozen and thawed swine heart mitochondria. Simultaneous oxidation of NADH and succinate showed complete additivity under a variety of experimental conditions, suggesting that the electron fluxes originating from NADH and succinate are completely independent and do not mix at the level of the so-called mobile diffusible components. We ascribe the results to mixing of the fluxes at the level of cytochrome c in bovine mitochondria: the Complex IV flux...
Source: Biochimica et Biophysica Acta - April 14, 2023 Category: Biochemistry Authors: Salvatore Nesci Cristina Algieri Fabiana Trombetti Micaela Fabbri Giorgio Lenaz Source Type: research

Cadmium exposure induces mitochondrial pathway apoptosis in swine myocardium through xenobiotic receptors-mediated CYP450s activation
In this study, the cardiotoxicity of Cd(II) was investigated by establishing a Cd(II)-exposed swine model (CdCl2, 20 mg Cd/Kg diet). Terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) assay and transmission electron microscope were used to observe the apoptosis. Antioxidant capacity was evaluated by free radicals contents and antioxidant enzymes activities. RT-PCR and western blot were used to measure the expression of XRs, CYP450s and apoptosis-related genes. Our results revealed that Cd(II) exposure activated the XRs and increased the CYP450s expression, contributing to the production of reactive ox...
Source: Journal of Inorganic Biochemistry - February 13, 2021 Category: Biochemistry Authors: Xia Zhao Xiaojing Li Shengchen Wang Zijiang Yang Honggui Liu Shiwen Xu Source Type: research

Role of OGDH in Atophagy-IRF3-IFN- β pathway during classical swine fever virus infection
In this study, we found that CSFV significantly reduces OGDH protein levels and promotes α-KG secretion through OGDH in PK-15 cells. Furthermore, we observed CSFV C protein interacts with OGDH and revealed that CSFV utilizes NDP52/NBR1 to target OGDH protein degradation in the autophagy-lysosome pathway. We also unveiled that OGDH overexpression inhibits CSFV proliferation, whereas OGDH knockdown increases CSFV proliferation. Further investigation into the mechanisms of OGDH on CSFV replication revealed that OGDH regulates the AMPK-mTOR-autophagy pathway. Additionally, using the autophagy agonist/inhibitor, rapamycin/3-MA...
Source: International Journal of Biological Macromolecules - August 21, 2023 Category: Biochemistry Authors: Sen Zeng Wenhui Zhu Zipeng Luo Keke Wu Zhimin Lu Xiaowen Li Weijun Wang Wenshuo Hu Yuwei Qin Wenxian Chen Lin Yi Shuangqi Fan Jinding Chen Source Type: research

Kinetics of Methane Production from Swine Manure and Buffalo Manure.
Abstract The degradation kinetics of swine and buffalo manure for methane production was investigated. Six kinetic models were employed to describe the corresponding experimental data. These models were evaluated by two statistical measurements, which were root mean square prediction error (RMSPE) and Akaike's information criterion (AIC). The results showed that the logistic and Fitzhugh models could predict the experimental data very well for the digestion of swine and buffalo manure, respectively. The predicted methane yield potential for swine and buffalo manure was 487.9 and 340.4 mL CH4/g volatile solid (VS)...
Source: Applied Biochemistry and Biotechnology - August 16, 2015 Category: Biochemistry Authors: Sun C, Cao W, Liu R Tags: Appl Biochem Biotechnol Source Type: research

Vitamin D attenuates HMGB1-mediated neointimal hyperplasia after percutaneous coronary intervention in swine.
In this study, we examined the association of vitamin D status with high mobility group box 1 (HMGB1)-mediated pathways (HMGB1, receptor for advanced glycation end products [RAGE], and Toll-like receptor-2 and -4 [TLR2 and TLR4]) in neointimal hyperplasia in atherosclerotic swine following bare metal stenting. Yucatan microswine fed with a high-cholesterol diet were stratified to receive vitamin D-deficient (VD-DEF), vitamin D-sufficient (VD-SUF), and vitamin D-supplemented (VD-SUP) diet. After 6 months, PTCA (percutaneous transluminal balloon angioplasty) followed by bare metal stent implantation was performed in the lef...
Source: Molecular and Cellular Biochemistry - July 31, 2020 Category: Biochemistry Authors: Satish M, Gunasekar P, Asensio JA, Agrawal DK Tags: Mol Cell Biochem Source Type: research

Rapid and accurate detection of African swine fever virus by DNA endonuclease-targeted CRISPR trans reporter assay.
In this study, we developed a rapid and accurate ASFV-detection method based on the DNA endonuclease-targeted CRISPR trans reporter (DETECTR) assay. By combining recombinase polymerase amplification with CRISPR-Cas12a proteins, the DETECTR assay demonstrated a minimum detection limit of eight copies with no cross reactivity with other swine viruses. Clinical blood samples were detected by DETECTR assay and showed 100% (30/30) agreement with real-time polymerase chain reaction assay. The rapid and accurate detection of ASFV may facilitate timely eradication measures and strict sanitary procedures to control and prevent the ...
Source: Acta Biochimica et Biophysica Sinica - November 17, 2020 Category: Biochemistry Authors: Li Z, Wei J, Di D, Wang X, Li C, Li B, Qiu Y, Liu K, Gu F, Tong M, Wang S, Wu X, Ma Z Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Selenium deficiency causes apoptosis through endoplasmic reticulum stress in swine small intestine
This study was to explore whether Se deficiency can cause ER stress and induce apoptosis in swine small intestine. We established the Se deficiency swine model in vivo and the intestinal epithelial (IPEC-J2) cell Se deficiency model in vitro. The results of morphological observation showed that Se deficiency caused structural damage in intestinal villi and the decrease of goblet cell structure. The apoptotic characteristics such as nucleolar condensation, mitochondrial swelling, and apoptotic bodies were observed in the IPEC-J2 cells. The results of acridine orange/ethidium bromide and mitochondrial membrane potential fluo...
Source: BioFactors - June 15, 2021 Category: Biochemistry Authors: Yingying Zheng, Bo Zhang, Haoyue Guan, Xing Jiao, Jie Yang, Jingzeng Cai, Qi Liu, Ziwei Zhang Tags: Research Communication Source Type: research

Development and characterization of monoclonal antibodies against the N-terminal domain of African swine fever virus structural protein, p54
Int J Biol Macromol. 2021 Mar 15:S0141-8130(21)00576-6. doi: 10.1016/j.ijbiomac.2021.03.059. Online ahead of print.ABSTRACTAfrican swine fever virus (ASFV), a re-emerging DNA virus, causes a highly contagious disease for domestic pigs. It is running rife worldwide and threatening the global swine industry. Protein p54 is an attractive candidate for ASFV diagnostic and vaccine design. In this work, we designed a peptide to mimic the N-terminal domain (NTD) of ASFV p54 and pretested it with sera from ASFV-infected pigs. The peptide could be well recognized by the sera, implying that the NTD of p54 contained some potential li...
Source: International Journal of Biological Macromolecules - March 19, 2021 Category: Biochemistry Authors: Aiping Wang Min Jiang Hongliang Liu Yankai Liu Jingming Zhou Yumei Chen Peiyang Ding Yanwei Wang Weisheng Pang Yanhua Qi Gaiping Zhang Source Type: research

A novel linear epitope at the C-terminal region of the classical swine fever virus E2 protein elicits neutralizing activity
In this study, Balb/c mice were immunized with plant-derived E2 protein, and four monoclonal antibodies (mAbs) 4B11, 7B3, 11A5 and 6F3 were generated. Two of these mAbs, 4B11 and 7B3, effectively blocked CSFV infection of PK-15 cells. Both mAbs recognized a novel linear epitope, 256CLIGNTTVKVHASDER271. The neutralizing ability of anti-CSFV serum decreased 63%, when pre-incubated with the linear peptide at 200 μg/mL. Structural analysis showed that this linear epitope is present at the border of Domain C and Domain D on the surface of the E2 protein. Alignment of amino acid sequences showed that the epitope was conserved i...
Source: International Journal of Biological Macromolecules - August 17, 2021 Category: Biochemistry Authors: Qianru Xu Junqing Guo Fanshu Ma Linke Liu Yanan Wang Shenli Zhang Xiangxiang Niu Xueyang Li Min Jiang Yanwei Wang Li Wang Yunchao Liu Qingmei Li Shujun Chai Ruining Wang Qiang Ma Erqin Zhang Gaiping Zhang Source Type: research